Protein expression and purification services are vital for research in many areas. The proteins produced must be separated from contaminants to allow detailed structural and biochemical characterization. Regardless of the complexity of your project, our experienced team has a wide range of tools to meet all your protein needs. Our expertise in cloning techniques and our range of chromatography systems and columns are essential for efficient protein separation, analysis, and purification. We also have a wide range of centrifuges, electrophoresis apparatus and spectrophotometers to ensure consistent quality and accurate results.

Proteins are complex mixtures and can be in a variety of states in the cell. To separate one particular protein from other proteins, cells are broken open using methods such as mechanical disruption (cell lysis), chemical lysis with detergents and enzymes or sonication. The resulting mixture is called the lysate and it contains the target protein along with other cellular components. The lysate is then further processed to obtain a highly pure product, often requiring multiple steps to remove salt and other impurities.

Western Blotting Service: High-Quality Protein Detection and Analysis

In the chromatography process, proteins are separated into different fractions based on their affinity for different liquid phases. The most common first step is an affinity chromatography step to isolate the desired protein. In some cases, the protein may be attached to an affinity tag that can then be removed with a specific protease. Other chromatography techniques include ion exchange and hydrophobic interaction chromatography. In addition, a dialysis method can be used to remove small molecules such as salts or other low molecular weight impurities from a protein solution by passing the sample through a semipermeable membrane.